Figure 2 – Animal Usage and Experimental Protocols
A) Schematic showing the various arteries harvested from each rat.
B) Each rat will contribute to several experiments performed by three experimentalists (one technician and two postdoctoral research associates). For example, endothelium dependent relaxation will be assessed in pressurized arteries, whilst endothelial cell Ca2+ signalling and regeneration is investigated in en face artery preparations.
C) Example protocols for studying endothelial cell regeneration. Temporal investigations requiring fixation protocols necessitate a multi-timepoint experimental design.