Distinct Endothelial Cell Clusters

Spatially distinct cell clusters activated by the EC25 concentrations of acetylcholine and histamine. The upper panels show the spatially-distinct clusters of cells that respond to the EC25 of acetylcholine (green; left) and histamine (magenta; right) in the same preparation. The lower panel shows an overlay of the responses to acetylcholine (green) and histamine (magenta) indicating the separate populations of cells activated by each agonist. Scale bars, 50 µm.