Dr Charlotte B. Buckley MPhys, MRes, PhD
Post Doctoral Research Associate
Charlotte started her scientific career by completing a undergraduate Masters in Physics at the University of Durham, where she became interested in the use of optics for helping to solve biological problems.
She then gained a position on the BHF Centre of Research Excellence 4 Year PhD Program at Edinburgh University, which aimed to integrate physical scientists into the biological sciences. During her PhD with Prof John Mullins, she used state-of-the-art imaging, image analysis and molecular biology techniques to try and understand how the enzyme renin, which is important for blood pressure regulation, is formed, processed and secreted.
She did her first post doctoral position at the University of Edinburgh on characterisation and optimisation of precision cell targeting in larval zebrafish by integrating a Bessel beam into a home-built selective plane illumination microscope.
She also worked in the Confocal and Light Microscopy imaging facility, helping researchers achieve their scientific objectives and providing training on widefield, confocal, multiphoton and light sheet systems.
Charlotte moved to Strathclyde and took up her position as a Post Doctoral Research Associate in the McCarron group in April 2018. In addition to using novel microscopy techniques to rapidly image the vascular smooth muscle and endothelial cell layers in en face endothelial preparations, Charlotte is investigating the effect of immunosuppressant drugs on calcium signalling in the intact endothelium.
Charlotte is committee chair of the Scottish Microscopy Society.
Charlotte can be contacted via:
Lee MD, Buckley C, Zhang, X, Louhivuori, L, Uhlen, P, Wilson, C, McCarron, JG, 2022, 'Small-world connectivity dictates collective endothelial cell signaling'. PNAS. DOI: 10.1073/pnas.2118927119
Hoffman S, Mullins L, Rider S, Brown C, Buckley C, Assmus A, Li Z, Betlran MS, Henderson N, del Pozo J, Martini ADG, Sequeira-Lopez MLS, Gomez RA, Mullins J, 2022, 'Comparative Studies of Renin-Null Zebrafish and Mice Provide New Functional Insights'. Hypertension. DOI: 110.1161/HYPERTENSIONAHA.121.18600
Zhang, X, Lee MD, Buckley C, Wilson, C, McCarron, JG, 2021, 'Mitochondria regulate TRPV4 mediated release of ATP'. British Journal of Pharmacology. DOI: 10.1111/bph.15687
Ivy JR, Carter RN, Zhao JF, Buckley, C, ....., Chapman KE, 2021, 'Glucocorticoids regulate mitochondrial fatty acid oxidation in fetal cardiomyocytes'. Journal of Physiology. DOI: http://doi.org/10.1113/JP281860
Buckley, C, 'Total internal reflection fluorescence microscopy'. Imaging Modalities for Biological and Preclinical Research: A compendium, Volume 1, IoP Science, Pages 1.3a-1 to 1.3.a-9
Buckley, C, Zhang, X, Wilson, C, McCarron, JG, 2020, 'Carbenoxolone and 18b-glycyrrhetinic acid inhibit IP3-mediated endothelial cell calcium signalling and depolarise mitochondria'. British Journal of Pharmacology. DOI: 10.1111/bph.15329
Kaveh, A, Bruton, FA, Buckley, C, Oremek, M, Tucker, CS, Mullins, JJ, Taylor, JM, Rossi, AG, Denvir, MA, 2020, 'Live imaging of heart injury in larval zebrafish reveals a multi-stage model of neutrophils and macrophage migration'. Frontiers in Cell and Developmental Biology. DOI: 10.3389/fcell.2020.579943
Wilson, C, Zhang, X, Lee, MD, MacDonald, M, Heathcote, HR, Alorfi, NMN, Buckley, C, Dolan, S, McCarron, JG 2019, 'Disrupted endothelial cell heterogeneity and network organization impair vascular function in prediabetic obesity'. Metabolism. DOI: 10.1016/j.metabol.2020.154340
Buckley, C, Wilson, C, McCarron, JG 2019, 'FK506 regulates IP3-evoked Ca2+ release independently of FKBP in endothelial cells'. British Journal of Pharmacology. DOI: 10.1111/bph.14905
Taylor, JM, Nelson, CJ, Bruton, FA, Baghbadrani, AK, Buckley, C, Tucker, CS, Rossi, AG, Mullins, JJ, Denvir, MA 2019, 'Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart'. Nature Communiation. DOI: 10.1038/s41467-019-13112-6
Lawton, PF, Buckley, C, Saunter CD, Wilson, C, Corbett AD, Salter PS, McCarron, JG, Girkin JM 2019, 'Multi-plane remote refocusing epifluorescence microscopy to image dynamic Ca2+ events'. Biomedical Optics Express. DOI: 10.1364/BOE.10.005611
Wilson, C, Zhang, X, Buckley, C, Heathcote, H, Lee, MD, McCarron, JG 2019, 'Increased Vascular Contractility in Hypertension Results From Impaired Endothelial Calcium Signaling' Hypertension. DOI: 10.1161/HYPERTENSIONAHA.119.13791
McCarron, JG, Wilson, C, Heathcote, H, Zhang, X, Buckley, C, and Lee, MD (2019). Heterogeneity and emergent behaviour in the vascular endothelium. Current Opinions in Pharmacology DOI: 10.1016/j.coph.2019.03.008
Wilson, C, Lee, MD, Heathcote, H, Zhang, X, Buckley, C, Girkin, JM, Saunter, CD, McCarron, JG 2019, 'Mitochondrial ATP production provides long-range control of endothelial inositol trisphosphate–evoked calcium signaling' Journal of Biological Chemistry. DOI: 10.1074/jbc.RA118.005913
Hoffmann, S, Mullins, LJ, Buckley, C, Rider, S & Mullins, JJ 2018, 'Investigating the RAS can be a fishy business: interdisciplinary opportunities using Zebrafish', Clinical Science. DOI: 10.1042/CS20180721
Buckley, C, Nelson, RJ, Mullins, LJ, Sharp, MG, Fleming, S, Kenyon, CJ, Semprini, S, Steppan, D, Peti-Peterdi, J, Kurtz, A, Christian, H & Mullins, JJ 2018, 'Phenotypic dissection of the mouse Ren-1(d) knockout by complementation with human renin' Journal of Biological Chemistry. DOI: 10.1074/jbc.RA117.000160
Buckley, C, Carvalho, MT, Young, LK, Rider, SA, McFadden, C, Berlage, C, Verdon, RF, Taylor, JM, Girkin, JM & Mullins, JJ 2017, 'Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish' Scientific Reports, vol 7, no. 1, pp. 5096. DOI: 10.1038/s41598-017-05028-2
Buckley, C, Dun, AR, Peter, A, Bellamy, C, Gross, KW, Duncan, RR & Mullins, JJ 2017, 'Bimodal dynamics of granular organelles in primary renin-expressing cells revealed using TIRF microscopy' American Journal of Physiology-Renal Physiology, vol 312, no. 1, pp. F200-F209. DOI: 10.1152/ajprenal.00384.2016
Vliegenthart, A.D.B., Wei, C., Buckley, C., Berends, C., de Potter, C.M., Schneemann, S., Del Pozo, J., Tucker, C., Mullins, J.J., Webb, D.J. and Dear, J.W., 2017. Characterization of triptolide-induced hepatotoxicity by imaging and transcriptomics in a novel zebrafish model. Toxicological Sciences, 159(2), pp.380-391. DOI: 10.1093/toxsci/kfx144
Stefanska, A, Kenyon, C, Christian, HC, Buckley, C, Shaw, I, Mullins, JJ & Péault, B 2016, 'Human kidney pericytes produce renin' Kidney International. DOI: 10.1016/j.kint.2016.07.035
Saunter, C.D., Semprini, S., Buckley, C., Mullins, J. and Girkin, J.M., 2012. Micro-endoscope for in vivo widefield high spatial resolution fluorescent imaging. Biomedical optics express, 3(6), pp.1274-1278. DOI: 10.1364/BOE.3.001274
Buckley C, L'Huillier N, Mullins L, Semprini S, Christian H, Mullins JJ, 2022, 'Late Fetal and Newborn Granulopoiesis but not Active Renin is Increased by Maternal Captopril Treatment During Perinatal Kidney Development'. Preprint, DOI: 10.1101/2022.05.15.491999