Prof. John McCarron likes mountains, and lies about mountains (it'll be fun he said), and likes going up mountains. He loves hills that much he's almost over one...Prof. John McCarron likes mountains, and lies about mountains (it'll be fun he said), and likes going up mountains. Prof. John McCarron likes mountains, and lies about mountains (it'll be fun he said), and likes going up mountains. Prof. John McCarron likes mountains, and lies about mountains (it'll be fun he said), and likes going up mountains. Prof. John McCarron likes mountains, and lies about mountains (it'll be fun he said), and likes going up mountains. Prof. John McCarron likes mountains, and lies about mountains (it'll be fun he said), and likes going up mountains. Prof. John McCarron likes mountains, and lies about mountains (it'll be fun he said), and likes going up mountains.
In his spare time, John likes going up mountains.
Outwardly propagating Ca wave propagation is not blocked by localized BAPTA/AM Ca buffering
En face mesenteric artery stained with Cal520/AM (5 µM, grey) and cIP (5 μM). Ca activity was stimulated by localized photolysis of cIP (yellow circle) after a baseline recording (5 Hz per channel; baseline cut short for the purpose of video). In the same preparation, the resulting Ca activity is visualized in green before (control) and after localized BAPTA/AM (30 µM, 15 mins plus 15 mins rest) application using pressure ejection from a puffer pipette, determined using sulforhodamine B (1 µM) signal (magenta application region). Imaging was performed in the presence of counter-propagating PSS flow (1.5 ml.min-1). Scale bar: 50 μm.
2+
2+
2+
2+
3
3