Network patterns are unique for each activator.
The video shows a field of native endothelial cells (mesenteric artery) loaded with a fluorescence calcium indicator. In the same artery, endothelial calcium activity was stimulated by acetylcholine (ACh) or TRPV4 activator (GSK). The intercellular waves evoked by GSK appear more coordinated than those evoked by ACh. Top panel: visualisation of change in fluorescence intensity; Middle panel: 5 randomly chosen individual cellular Ca2+ responses; Lower panel: heat plot showing the Ca2+ activity of all cells in the field of view. Each line represents an individual cell.