Network Patterns evoked by Carbachol and ATP
Network patterns are unique for each activator.
The video shows a field of native endothelial cells (mesenteric resistance artery) loaded with a fluorescence calcium indicator. In the same artery, endothelial calcium activity was stimulated by carbachol (CCh) and ATP. Different cells were activated by each activator. Top panel: visualisation of change in fluorescence intensity and maximum intensity image showing all activated cells; Middle panel: 5 randomly chosen individual cellular Ca2+ responses; Lower panel: heat plot showing the Ca2+ activity of all cells in the field of view. Each line represents an individual cell.